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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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The diurnal rhythm of liver glycogen phosphorylase: correlating changes in enzyme activity and enzymic protein.

The diurnal rhythm of phosphorylase activity in mouse liver extracts was correlated with the 24 h fluctuations in phosphorylase protein. This last measurement was made using rocket immunoelectrophoresis. The peak activity of phosphorylase appeared coincident (at 18:00 h) with the greatest amount of phosphorylase protein detected. Conversely, the lowest activity measured and lowest enzymic protein content both occurred at 02:00 h. Regression analysis revealed a significant positive correlation between enzyme activity and protein. Thus changes in the cellular concentration of this enzyme are implicated in the diurnal rhythm of liver glycogen.[1]

References

  1. The diurnal rhythm of liver glycogen phosphorylase: correlating changes in enzyme activity and enzymic protein. Roesler, W.J., Khandelwal, R.L. FEBS Lett. (1986) [Pubmed]
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