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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Rapid method for determination of kanamycin and dibekacin in serum by use of high-pressure liquid chromatography.

A rapid, simple, and accurate method for the determination of kanamycin and dibekacin in serum by use of high-pressure liquid chromatography is described. The serum proteins were precipitated with 3.5% perchloric acid containing sodium octanesulfonate. After centrifugation, a sample of the supernatant was directly injected into the chromatograph. The determination of kanamycin and dibekacin was performed by a combination of reverse-phase, ion-pair chromatography, postcolumn derivatization with o-phthalaldehyde, and fluorescence detection. The correlation coefficients with fluorescence polarization immunoassay were 0.996 for kanamycin and 0.957 for dibekacin.[1]

References

  1. Rapid method for determination of kanamycin and dibekacin in serum by use of high-pressure liquid chromatography. Kubo, H., Kobayashi, Y., Nishikawa, T. Antimicrob. Agents Chemother. (1985) [Pubmed]
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