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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A new method for excitation-contraction uncoupling in frog skeletal muscle.

The mechanical activity of frog sartorius muscle fibers can be uncoupled from the electrical activity of their surface membranes by immersing the preparation in Ringer solution containing either 1.5 or 2.0 M of formamide for 15--20 min. This uncoupling is not reversed when the muscle is transferred to normal frog Ringer solution. Formamide does not affect the electrical activity of the sciatic nerve branch, and both endplate potentials and miniature endplate potentials may be recorded from the uncoupled muscles. Prolonged exposure to formamide, beyond the time needed to paralyze, causes neuromuscular block.[1]

References

  1. A new method for excitation-contraction uncoupling in frog skeletal muscle. del Castillo, J., Escalona de Motta, G. J. Cell Biol. (1978) [Pubmed]
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