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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Cloning and simplified purification of Escherichia coli DNA gyrase A and B proteins.

We have transferred the Escherichia coli gyrA and gyrB genes onto plasmids that allow the overproduction of the DNA gyrase A and B proteins and have designed relatively simple purification procedures for both proteins. The pure proteins are obtained in good yield; from 2 liters of culture (12 g of cells), one can recover 25 mg of GyrA or 3 mg of GyrB protein.[1]

References

  1. Cloning and simplified purification of Escherichia coli DNA gyrase A and B proteins. Mizuuchi, K., Mizuuchi, M., O'Dea, M.H., Gellert, M. J. Biol. Chem. (1984) [Pubmed]
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