- Affinity purification of newly phosphorylated protein molecules. Thiophosphorylation and recovery of histones H1, H2B, and H3 and the high mobility group protein HMG-1 using adenosine 5'-O-(3-thiotriphosphate) and cyclic AMP-dependent protein kinase. Sun, I.Y., Johnson, E.M., Allfrey, V.G. J. Biol. Chem. (1980)