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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Quantitative determination of tiamenidine hydrochloride in human serum using gas chromatography mass spectrometry.

A method has been developed for the blood level determination of the antihypertensive agent tiamenidine hydrochloride. The serum samples are mixed with deuterium labelled tiamenidine hydrochloride as an internal standard and extracted with methylene chloride. The extracts are derivatized with heptafluorobutyric acid anhydride and analysed by means of gas chromatography mass spectrometry using the selected ion monitoring technique to measure the molecular ion intensities of the bis-heptafluorobutyryl derivatives of tiamenidine hydrochloride and of the internal standard. Using 5 ml serum, the limit of detection is 0.2 ng ml-1 with an accuracy of +/- 0.17 ng (Syx of the calibration curve).[1]

References

  1. Quantitative determination of tiamenidine hydrochloride in human serum using gas chromatography mass spectrometry. Fehlhaber, H.W., Metternich, K., Tripier, D., Uihlein, M. Biomed. Mass Spectrom. (1978) [Pubmed]
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