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Cutler, A.J. et al.

Quantitation of the delivery of liposome contents into plant protoplasts.

A procedure for the quantitation of the delivery of liposome contents into Catharanthus roseus protoplasts has been developed. The method is based on the uptake of liposome encapsulated methylumbelliferyl beta-D-glucoside and its enzymatic hydrolysis to yield fluorescent methylumbelliferone. Since the free glucoside is not taken up by the protoplasts to a significant extent, the delivery of material in the nanomole range can be measured with ease.[1]

References

Quantitation of the delivery of liposome contents into plant protoplasts. Cutler, A.J., Constabel, F., Kurz, W.G., Shargool, P.D. Anal. Biochem. (1984) [Pubmed]

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