HPLC assay for 2-(3-aminopropylamino)ethanethiol (WR-1065) in plasma.
A high pressure liquid chromatography (HPLC) plasma assay for WR-1065 is described which is both precise (coefficient of variation less than 5%) and accurate (% average deviation less than or equal to 6.1) throughout the concentration range from 1 to 500 micrograms/mL of plasma. The analyte is separated by HPLC and detected with a thiol specific electrochemical transducer cell. The detector response is linear over the ranges 1 to 10 micrograms/mL (R2 = 0.995), 10 to 100 micrograms/mL (R2 = 0.995), and 100 to 500 micrograms/mL (R2 = 0.974). The absolute retention times for WR-1065 and WR-1729 are 9 and 12 minutes, respectively. The assay uses 100 microL of plasma and requires a total chromatography cycle time of 40 minutes. The method has been found suitable for the determination of WR-1065 in plasma from a beagle dog after i.v. administration of S-2-(3-aminopropylamino)ethyl phosphorothioate (WR-2721).[1]References
- HPLC assay for 2-(3-aminopropylamino)ethanethiol (WR-1065) in plasma. McGovern, E.P., Swynnerton, N.F., Steele, P.D., Mangold, D.J. Int. J. Radiat. Oncol. Biol. Phys. (1984) [Pubmed]
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