Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Lakings, D.B. et al.

Quantitative determination of N-(trans-2-dimethylaminocyclopentyl)-N-(3',4'-dichlorophenyl) propanamide and its N-demethyl metabolite in dog serum by gas chromatography.

A gas chromatographic-electron capture (GC-EC) method has been developed for the determination of N-(trans-2-dimethylaminocyclopentyl)-N-(3',4'-dichlorophenyl)pr opanamide, a potential antidepressant drug, and its N-demethyl metabolite in serum. The GC-EC system employed a 3% OV-17 on 100/120 mesh Supelcoport, 2-m X 2-mm i.d. glass column and an isothermal temperature of 195 degrees C. The parent drug and metabolite were extracted from alkalinized serum (pH approximately 13) with toluene, back-extracted into an acidic solution (pH approximately 1), and finally, after adjusting to pH 13, extracted again with toluene. The extensive sample cleanup was necessary to remove serum components which interfered with the analysis. The analytical method was shown to give quantitative recovery of the drug and metabolite, to be linear over a 100-fold concentration range, and to have the necessary precision and sensitivity to detect and quantify as little as 1 ng/mL of the drug or its metabolite. The method has been employed to determine the serum level of drug and metabolite in dogs receiving a single oral dose and to determine the possible correlation between the administered dose and serum levels.[1]

References

Quantitative determination of N-(trans-2-dimethylaminocyclopentyl)-N-(3',4'-dichlorophenyl) propanamide and its N-demethyl metabolite in dog serum by gas chromatography. Lakings, D.B., Stryd, R.P., Gilbertson, T.J. Journal of pharmaceutical sciences. (1984) [Pubmed]

Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.