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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

An improved gas-liquid chromatographic method for the determination of fecal neutral sterols.

The analysis of fecal neutral sterols has been improved by use of a highly selective gas-liquid chromatography column packed with SP-2401. This chromatographic column allows separation of cholesterol and cholestanol and delta 5-5 alpha plant sterol homologs without employing silver nitrate thin-layer chromatography. Furthermore, there is no need to derivatize neutral sterols before injection. The main fecal neutral sterols are well resolved; retention times are reproducible; detector response is reproducible, linear, and sensitive to 0.2 micrograms. This method, successfully used for fecal samples, may be suggested as a routine method for the clinical study of cholesterol metabolism.[1]

References

  1. An improved gas-liquid chromatographic method for the determination of fecal neutral sterols. Arca, M., Montali, A., Ciocca, S., Angelico, F., Cantafora, A. J. Lipid Res. (1983) [Pubmed]
 
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