A new plastic for morphometric investigation of blood vessels, especially in large organs such as the human liver.
In this paper a technique is described, using Araldite CY 223 and hardener HY 2967 as injection material, for preparing corrosion casts or histological sections. The plastic has a viscosity (at 39-40 degrees C) similar to that of blood, a gelling time of approximately 17 min (at 40 degrees C), and an exothermic transition energy of delta H = 80.28 +/- 3.20 cal/gm. The influence of the plastic on the tissue is discussed. The histological sectioning of fixed tissue containing Araldite-filled blood vessels after embedding in 2-hydroxyethyl-methacrylate ( GMA) is described. When using GMA in a modification of the mixtures of Ruddell (1967) and Sims (1974), methylbenzoate is recommended as an intermedium in order to obtain a more uniform infiltration and reproducible section thickness. At the same time methylbenzoate is recommended as a storing fluid. Sections of 2-3 micrometers afford satisfying morphologic and morphometric results. This method allows various arterial wall dimensions to be measured easily, and provides a suitable means to compare histometric values with SEM data derived from corrosion casts.[1]References
- A new plastic for morphometric investigation of blood vessels, especially in large organs such as the human liver. Hanstede, J.G., Gerrits, P.O. Anat. Rec. (1982) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
