Physiological requirements for ciliary reactivation of bracken fern spermatozoids.
Physiological parameters affecting reactivated ciliary beat in spermatozoids of braken fern (Pteridium aquilinum) were studied using a Triton/glycerol permeabilized cell model system. Reactivation frequencies of polylysine-tethered cells equalled in vivo rates at neutral pH. Frequency was dependent on ATP and Mg2+ concentration, and reactivation was inhibited by millimolar or greater free calcium. Reactivation was reversibly inhibited by micromolar concentrations of sodium ortho-vanadate, while intact cells were not affected by millimolar levels of the inhibitor. This is the first characterization of in vitro ciliary beat in a non-algal plant cell and demonstrates that the nucleotide and ionic requirements for reactivation of bracken cilia are similar to those of other systems.[1]References
- Physiological requirements for ciliary reactivation of bracken fern spermatozoids. Wolniak, S.M., Cande, W.Z. J. Cell. Sci. (1980) [Pubmed]
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