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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Intracellular distribution of NADP-linked isocitrate dehydrogenase, fumarase and citrate synthase in bovine heart muscle.

By fractional extraction of minced bovine heart muscle with iso-osmotic sucrose and phosphate buffer solutions, it is shown that less than 4% of the total citrate synthase in the tissue is in the cytosol. Using citrate synthase as a marker for broken mitochondria, two methods of fractionation of 750 x g supernatants from homogenates of bovine heart muscle show that 10% of the total fumarase and NADP-linked isocitrate dehydrogenase activities are present in the cytoplasm. Homogenates prepared by sonication and osmotic shock and by sand-grinding gave closely similar results as regards enzyme distributions and extent of mitochondrial breakage. The results are compared with those reported for other tissues.[1]

References

  1. Intracellular distribution of NADP-linked isocitrate dehydrogenase, fumarase and citrate synthase in bovine heart muscle. Fatania, H.R., Dalziel, K. Biochim. Biophys. Acta (1980) [Pubmed]
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