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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Recombinant f1 phage-mediated transfection of mammalian cells using lipopolyamine technique.

Recombinant f1 phages carrying a shuttle vector pKA1M for expression of blasticidin S deaminase were introduced into monkey COS7 cells by mixing with dioctadecylamidoglycylspermine (DOGS). Blasticidin S selection resulted in the detectable growth of resistant colonies within a week. The transfection efficiency depended on the amounts of the phage and DOGS, their ratio, and the time during which the cells were incubated with the phage/DOGS mixture. This method requires only several microliters of an Escherichia coli culture medium containing recombinant f1 phage particles and is applicable to various cell lines including mouse NIH/3T3, chinese hamster CHO-K1, and human HT-1080.[1]

References

  1. Recombinant f1 phage-mediated transfection of mammalian cells using lipopolyamine technique. Yokoyama-Kobayashi, M., Kato, S. Anal. Biochem. (1994) [Pubmed]
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