The high non-enzymatic conjugation rates of some glutathione S-transferase (GST) substrates at high glutathione concentrations.
Enzymatic properties of five human glutathione S-transferases (GSTs) and non-enzymatic conjugation rates of GST substrates were examined as a function of glutathione (GSH) concentration and pH. GSTP1-1 showed a broad substrate specificity with both low and high GSH (10 mM) concentrations at pH 7.0, and the inhibitor insensitivity was then prominent. Among the GST substrates tested, ethacrynic acid, p-nitrophenylacetate, 1-chloro-2,4-dinitrobenzene and trans-4-phenyl-3-buten-2-one conjugated nonenzymatically with half-times of 0.39, 3.1, 9.4 and 10.0 min respectively at 10 mM GSH, pH 7.0 and 35 degrees C. The half-time for acrolein estimated by extrapolation was approximately 0.5 s. While the enzymatic reaction rates were independent of GSH at concentrations larger than Km values according to Michaelis-Menten kinetics and relatively insensitive to a pH change from 6.5 to 7.0, the non-enzymatic ones were linearly proportional to the GSH concentration and sensitive to pH. The enhanced non-enzymatic conjugation of various electrophiles at high GSH concentrations may in part account for the physiological significance of GSH elevation in preneoplastic and neoplastic cells.[1]References
- The high non-enzymatic conjugation rates of some glutathione S-transferase (GST) substrates at high glutathione concentrations. Satoh, K. Carcinogenesis (1995) [Pubmed]
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