The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Editor

Share

Personal info

View

Links

About

Terms

 

 
 
 
 

Microcalorimetric study of the binding of thiodigalactoside to the lactose permease M protein of Escherichia coli.

The energetics of the binding of thiodigalatoside onto vesicles of Escherichia coli containing M protein is described. The Kd determined from equilibrium dialysis was 5-10(-5) M. The enthalpy change (deltaH) was measured by calorimetry. The derived deltaG and deltaH values allowed estimation of the entropic change associated with the binding reaction. The control experiments were made with membranes from cells that were not induced for the lac system. All the experiments were carried out in presence of 10(-2) M sodium azide to prevent any concentration of thiodigalactoside into the vesicles. It was concluded that such membrane vesicles which are in a de-energized state are able to bine thiodigalactoside specifically with a Kd corresponding to the Km of the entry of beta-galactoside measured with intact, active cells.[1]

References

  1. Microcalorimetric study of the binding of thiodigalactoside to the lactose permease M protein of Escherichia coli. Belaich, A., Simonpietri, P., Belaich, J.P. J. Biol. Chem. (1976) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
 
WikiGenes - Universities