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Determination of 18 beta-glycyrrhetic acid in human plasma by high-performance liquid chromatography.

A sensitive high-performance liquid chromatographic method has been developed for the determination of 18 beta-Glycyrrhetic acid in human plasma. 18 alpha-Glycyrrhetic acid was used as an internal standard. The procedure includes pretreatment by liquid-liquid extraction and chromatographic separation on a reversed-phase column with ultraviolet detection. The detection limit of the drug was 5 ng/ml. The method was applied to the determination of 18 beta-glycyrrhetic acid in plasma after oral administration of a herbal medicine containing 18 beta-glycyrrhitic acid in human volunteers.[1]

References

  1. Determination of 18 beta-glycyrrhetic acid in human plasma by high-performance liquid chromatography. Abe, K., Suzuki, A., Katayama, H., Tatsumi, Y., Yumioka, E. J. Chromatogr. B, Biomed. Appl. (1994) [Pubmed]
 
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