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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Expression and crystallization of a soluble form of Drosophila fasciclin III.

A truncated form of Drosophila fasciclin III has been engineered by site-directed mutagenesis. Secreted fasciclin III is expressed at 35 to 40 mg/l in insect cells with baculovirus carrying the recombinant gene. Single crystals of purified soluble fasciclin III have been grown by vapor diffusion versus polyethylene glycol 8000/sodium citrate at low pH. The space group is P6(1)22 or its enantiomorph P6(5)22, with unit cell dimensions a = b = 140 A, c = 260 A. Cryo-preserved crystals diffract to reciprocal lattice spacings beyond 3.0 A.[1]

References

  1. Expression and crystallization of a soluble form of Drosophila fasciclin III. Strong, R.K., Vaughn, D.E., Bjorkman, P.J., Snow, P.M. J. Mol. Biol. (1994) [Pubmed]
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