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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Simultaneous genotyping of sex and kappa-casein of bovine in vitro fertilized embryos by the PCR technique.

Sex and the kappa-casein genotype of in vitro produced cattle demiembryos was identified by simultaneous PCR-genotyping using polymorphic sites in ZFY/ZFX and kappa-casein genes. We obtained PCR products of 530 bp (kappa-casein) and of 445/447 bp (sex). Sex and the two kappa-casein alleles could be identified after digestion of the PCR products with Pstl and simultaneous separation of the restriction fragments on one step, allowing a rapid and efficient analyses of embryos.[1]

References

  1. Simultaneous genotyping of sex and kappa-casein of bovine in vitro fertilized embryos by the PCR technique. Schellander, K., Mayr, B., Ertl, K., Peli, J. Zentralblatt für Veterinärmedizin. Reihe A. (1993) [Pubmed]
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