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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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In situ hybridization for the detection of chicken anemia virus in formalin-fixed, paraffin-embedded sections.

The development of a biotinylated in situ hybridization procedure for the detection of chicken anemia virus (CAV) is described. A double-stranded DNA probe was prepared using polymerase chain reaction and was biotinylated by nick translation. Hybridization conditions included the use of a microwave oven to denature target and probe nucleic acids and a five-step protocol for detection of biotinylated hybrids. In situ hybridization detected CAV nucleic acid in thymus tissue from experimentally infected birds after 6 hours, 3 days, and 7 days fixation time in formalin. However, immunocytochemical detection of CAV antigen was severely impaired if tissue was fixed in formalin for more than 6 hours.[1]

References

  1. In situ hybridization for the detection of chicken anemia virus in formalin-fixed, paraffin-embedded sections. Allan, G.M., Smyth, J.A., Todd, D., McNulty, M.S. Avian Dis. (1993) [Pubmed]
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