Biological and molecular properties of a pathotype P-1 and a pathotype P-4 isolate of pea seed-borne mosaic virus

J Gen Virol. 1996 Jun:77 ( Pt 6):1329-33. doi: 10.1099/0022-1317-77-6-1329.

Abstract

Two isolates of pea seed-borne mosaic potyvirus, DPD1 and NY, were identified as pathotypes P-1 and P-4, respectively, by their infectivity on Pisum sativum L. lines homozygous for the recessive resistance genes sbm-1 and sbm-4. The two isolates differed in several biological characteristics. DPD1 induced transient vein clearing, downward rolling of leaflets and internode shortening on P. sativum, whereas NY only caused a slight growth reduction. DPD1 moved systemically in Chenopodium quinoa whereas NY was restricted to inoculated leaves. DPD1 was frequently transmitted by seeds whereas NY was rarely seed-transmitted: 24% and 0.3%, respectively, in P. sativum '549'. Both DPD1 and NY were transmitted by aphids (Myzus persicae), though a DAG triplet was not present in the N terminus of the coat protein. The nucleotide sequence and deduced amino acid sequence of NY were determined and compared to the corresponding sequences of DPD1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Complementary
  • Genes, Plant
  • Genes, Recessive
  • Genome, Viral
  • Molecular Sequence Data
  • Open Reading Frames
  • Pisum sativum / virology*
  • Plant Diseases / genetics
  • Plant Diseases / virology
  • Polymerase Chain Reaction
  • Potyvirus / genetics*
  • Potyvirus / isolation & purification
  • Potyvirus / pathogenicity*
  • Seeds
  • Viral Proteins / genetics

Substances

  • DNA, Complementary
  • Viral Proteins

Associated data

  • GENBANK/X89997