The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

T-kinin is resistant to hydrolysis by angiotensin I-converting enzyme.

Several enzymes of are capable of cleaving kinins at either the N- or C-terminal end. In this work, we investigated the hydrolysis of T-kinin ( TK) and bradykinin (BK) by carboxypeptidase B ( CpB) and angiotensin I-converting enzyme (ACE). The kinins were incubated with the enzymes in Tyrode solution kept at 37 degrees C for 25 min. The kinin residual activity in the incubation medium was bioassayed on the isolated guinea pig ileum. BK (5 micrograms/ml) and TK (5 micrograms/ml) were rapidly and similarly degraded by CpB (0.088 mU/ml) since half of both kinins was inactivated within 5 min of incubation. At 25 min of incubation with CpB the TK and BK-like activities were 20.8 +/- 3.9 (1.04 +/- 0.20 micrograms/ml) and 31.8 +/- 5.0% (1.59 +/- 0.25 micrograms/ml) of the initial kinin concentrations, respectively. BK was also rapidly inactivated by ACE (5 mU/ml) while TK activity was unaffected. At 25 min the BK and TK residual activities corresponded to 39.4 +/- 2.5 (1.97 +/- 0.13 micrograms/ml) and 93.8 +/- 9.2% (4.69 +/- 0.46 micrograms/ml) of the initial kinin concentrations, respectively. It was concluded that TK, a kinin elongated at the N-terminal position, is resistant to ACE degradation.[1]

References

  1. T-kinin is resistant to hydrolysis by angiotensin I-converting enzyme. Passaglio, K.T., Vieira, M.A. Immunopharmacology (1996) [Pubmed]
 
WikiGenes - Universities