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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Cloning of an almost full-length chicken conalbumin double-stranded cDNA.

Chicken conalbumin double-stranded cDNA (con-dscDNA) was synthesized from a laying hen oviduct mRNA preparation enriched for conalbumin mRNA (con-mRNA). The dscDNA was inserted by blunt-end ligation into the Sal I site of plasmid pBR322 which had been repaired with DNA polymerase I to create Taq I sites on each side of the inserted fragment. After bacterial transformation, one hybrid recombinant, pBR322-con1, which contains the largest inserted dscDNA (about 2350 bp) was shown to hybridize specifically to the RNA which is translated into conalbumin. Electron microscopic examination of hybrid molecules between con-mRNA and pBR322-con1 DNA indicate that the inserted con-dscDNA is an almost full-length double-stranded transcript of conalbumin mRNA.[1]

References

  1. Cloning of an almost full-length chicken conalbumin double-stranded cDNA. Cochet, M., Perrin, F., Gannon, F., Krust, A., Chambon, P., McKnight, G.S., Lee, D.C., Mayo, K.E., Palmiter, R. Nucleic Acids Res. (1979) [Pubmed]
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