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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Bacterial production of transdihydroxycyclohexadiene carboxylates by metabolic pathway engineering.

Homochiral-cis-cyclohexa-3,5-diene-1,2-diols are important synthons. We found a way to produce trans-configured homochiral diols using recombinant Klebsiella pneumoniae 62-1. Transformation of this mutant (Phe- Trp- Tyr-) with plasmids carrying genes involved in chorismic and isochorismic acid metabolism leads to the production of either (+)-trans-(2S,3S)-2,3-dihydroxycyclohexa-4,6-dienecarboxylic acid or (-)-trans-(3R,4R)-3,4-dihydroxycyclohexa-1,5-dienecarboxylic acid, with a yield of 70 or 90 mg (1 culture broth)-1, respectively. The metabolic shift from one diene to the other is caused by a change in activity of isochorismate hydroxymutase and/or isochorismatase which in turn results from growth under iron deficiency or overexpression of genes ( entC and/or entB) involved in chrismate metabolism.[1]

References

  1. Bacterial production of transdihydroxycyclohexadiene carboxylates by metabolic pathway engineering. Müller, R., Breuer, M., Wagener, A., Schmidt, K., Leistner, E. Microbiology (Reading, Engl.) (1996) [Pubmed]
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