Novel expression and localization of active thrombomodulin on the surface of mouse brain astrocytes.
Thrombin's potent effects on astrocytes are mediated by a specific receptor and inhibited by a serpin, protease nexin I ( PNI). Thrombomodulin ( TM), a membrane protein that forms complexes with thrombin, changing its enzymatic specificity, has not been studied in astrocytes. In primary astrocyte cultures, using Western blotting and immunocytochemistry, we found a 70 kDa TM band and TM localized to the surface with an anti-mouse TM monoclonal antibody. By reverse transcriptase coupled with polymerase chain reaction (RT-PCR), we found the correct sequence for mouse TM mRNA in astrocytes. Finally, we documented calcium-dependent activation of protein C by a thrombin:TM complex with thrombin added to the astrocytes. These results indicate the presence of functionally active TM at the astrocyte surface and add support to a role for thrombin signaling in the nervous system.[1]References
- Novel expression and localization of active thrombomodulin on the surface of mouse brain astrocytes. Pindon, A., Hantai, D., Jandrot-Perrus, M., Festoff, B.W. Glia (1997) [Pubmed]
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