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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Transcript mapping in a 46-kb sequenced region at the core of 12q13.3 amplification in human cancers.

We used a combination of sequence analysis and exon trapping in an effort to determine the complete transcript map for a cosmid (6E5) derived from 12q13.3, a region of DNA sequence amplification in human cancers. This cosmid, previously known to contain three genes (CDK4, SAS, and OS9), was sequenced, and that information was used for computer-assisted analysis. In addition, 6E5 was subjected to both internal and 3'-terminal exon-trapping protocols, and the results of these studies were used to guide cDNA cloning experiments. These studies demonstrate that this cosmid is derived from a remarkably gene-dense region and add two new transcripts (KIAA0167 and 6E5.2) to the list of sequences that are expressed in tumors bearing amplification of this region.[1]

References

  1. Transcript mapping in a 46-kb sequenced region at the core of 12q13.3 amplification in human cancers. Elkahloun, A.G., Krizman, D.B., Wang, Z., Hofmann, T.A., Roe, B., Meltzer, P.S. Genomics (1997) [Pubmed]
 
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