Regulation of human prohormone convertase 2 promoter activity by the transcription factor EGR-1.
Prohormone convertases are involved in the tissue-specific endoproteolytic processing of prohormones and neuropeptide precursors within the secretory pathway. In the present study, we have isolated genomic clones comprising the 5'-terminal region of the human prohormone convertase 2 (PC2) gene and established characteristics of the PC2 promoter region. The proximal promoter region is very G+C-rich and does not contain a canonical TATA box or a CAAT box. Transient expression assays with a set of human PC2 gene fragments containing progressive 5' deletions demonstrate that the proximal promoter region is capable of directing high levels of neuroendocrine-specific expression of reporter gene constructs. In addition, we show that the transcription factor EGR-1 interacts with two distinct elements within the proximal human PC2 promoter region. Transfection experiments also demonstrate that EGR-1 is able to enhance PC2 promoter activity.[1]References
- Regulation of human prohormone convertase 2 promoter activity by the transcription factor EGR-1. Jansen, E., Ayoubi, T.A., Meulemans, S.M., Van De Ven, W.J. Biochem. J. (1997) [Pubmed]
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