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Martin-Esteban, A. et al.

Mixed immunosorbent for selective on-line trace enrichment and liquid chromatography of phenylurea herbicides in environmental waters.

An immunosorbent containing antiisoproturon and antichlortoluron antibodies immobilised on aldehyde-activated silica was employed for the on-line preconcentration and liquid chromatography-diode array detection of several phenylureas. The efficiency of the coupling of the immunosorbent to the liquid chromatographic system and the characteristics of the immunosorbent were evaluated. The on-line system allowed the selective trace enrichment of chlortoluron, isoproturon, metobromuron, linuron and chlorbromuron at the 0.05-0.5 microgram l-1 level in ground and river waters and provided detection limits in the range 0.01-0.03 microgram l-1 by percolating only 10 ml of water sample. The proposed method was validated by analysing freeze-dried tap water samples with a high content in pesticides of different chemical functionalities. The results obtained were compared with the mean value obtained in an interlaboratory exercise.[1]

References

Mixed immunosorbent for selective on-line trace enrichment and liquid chromatography of phenylurea herbicides in environmental waters. Martin-Esteban, A., Fernández, P., Stevenson, D., Cámara, C. The Analyst. (1997) [Pubmed]

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