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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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PCR for detection of Shigella spp. in mayonnaise.

The use of PCR to amplify a specific virA gene fragment serves as a highly specific and sensitive method to detect virulent bacteria of the genus Shigella and enteroinvasive Escherichia coli. Amplification of a 215-bp DNA band was obtained by using isolated genomic DNA of Shigella, individual cells of Shigella dysenteriae, and mayonnaise contaminated with S. dysenteriae. Moreover, a multiplex PCR with specific (virA) and bacterium-restricted (16S ribosomal DNA) primers generated an amplification product of approximately 755 bp for all bacteria tested and an additional 215-bp product for Shigella and enteroinvasive E. coli.[1]

References

  1. PCR for detection of Shigella spp. in mayonnaise. Villalobo, E., Torres, A. Appl. Environ. Microbiol. (1998) [Pubmed]
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