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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A high-copy-number ADE2-bearing plasmid for transformation of Candida glabrata.

The Candida glabrata ADE2 gene encoding aminoimidazole ribonucleotide (AIR) carboxylase (EC 4.1.1.21) was isolated by complementation of the ade2-1 mutation in Saccharomyces cerevisiae. The predicted amino acid (aa) sequence is 75% identical to that of S. cerevisiae. Integrative transformation was used to produce a C. glabrata strain bearing a deletion of ADE2 coding sequences. A high-copy-number shuttle vector bearing the ADE2 gene was constructed and contains a fragment of S. cerevisiae mitochondrial (mt) DNA that confers the ability to replicate autonomously in C. glabrata.[1]

References

  1. A high-copy-number ADE2-bearing plasmid for transformation of Candida glabrata. Hanic-Joyce, P.J., Joyce, P.B. Gene (1998) [Pubmed]
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