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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Two-dimensional chromatography in the analysis of complex glycans from transferrin.

Oligosaccharides released from human transferrin have been derivatized with 2-aminoacridone (2-AMAC) prior to analysis by either reversed- or normal-phase high-performance liquid chromatography. These separation methods are complementary and allow distinction between isomeric mono- and disialylated oligosaccharides, which terminate with Sia alpha 2-6Gal or Sia alpha 2-3Gal at the nonreducing end. Collected fractions of 2-AMAC-derivatized glycans were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, before and after desialylation.[1]

References

  1. Two-dimensional chromatography in the analysis of complex glycans from transferrin. Charlwood, J., Birrell, H., Tolson, D., Camilleri, P. Anal. Chem. (1998) [Pubmed]
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