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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Bioelectrochemical monitoring of phenols and aromatic amines in flow injection using novel plant peroxidases.

An amperometric flow system combined with a glucose oxidase-mutarotase reactor was optimized and used to determine aromatic amines and phenols using peroxidase-modified graphite electrodes. An increase in currents upon injection of the analyzed substrate was shown to be approximated by a Michaelis-Menten type dependence. The detection limit was calculated as 3 times the noise, and the sensitivity was calculated as Imax/K(m)app. Commercially available horseradish peroxidase was compared with tobacco anionic and peanut cationic peroxidases for determination of aromatic amines and phenols. Detection limits of 10 nM for determination of o-aminophenol and o- and p-phenylenediamine achieved with a tobacco peroxidase-modified electrode give a promise for further improvements in sensitivities and detection limits of biosensors.[1]

References

  1. Bioelectrochemical monitoring of phenols and aromatic amines in flow injection using novel plant peroxidases. Munteanu, F.D., Lindgren, A., Emnéus, J., Gorton, L., Ruzgas, T., Csŏregi, E., Ciucu, A., van Huystee, R.B., Gazaryan, I.G., Lagrimini, L.M. Anal. Chem. (1998) [Pubmed]
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