The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Electrical and optical monitoring of alpha-latrotoxin action at Drosophila neuromuscular junctions.

Electrophysiological recording demonstrates that alpha-latrotoxin, a 125,000 mol. wt component of black widow spider venom, promotes high frequency quantal discharges at larval neuromuscular junctions of Drosophila. Concomitantly, fluorescence imaging of presynaptic calcium ion activity reveals that this toxin qualitatively elevates cytosolic ionized calcium in this preparation. These activities of alpha-latrotoxin are selectively antagonized by a monoclonal antibody, 4C4.1, that was previously shown to inhibit the action of this toxin in PC-12 cells. However, 4C4.1 does not block the release-promoting activity of gel-filtered extracts of black widow spider venom. This indicates that black widow spider venom has multiple components that promote quantal transmitter secretion in invertebrates. This investigation demonstrates that alpha-latrotoxin is among the active principles in black widow spider venom that enhance transmitter release and raise cytosolic ionized calcium in Drosophila. These results suggest that Drosophila, because of the relative ease of genetic manipulation, may be useful to study the target protein(s) that mediate the binding and action of alpha-latrotoxin at nerve endings. Moreover, the procedure that we report for loading Drosophila nerve terminals with the calcium ion-sensing dye, Calcium Crimson, may have utility for studying calcium dynamics in mutant alleles with alterations in synapse development and function in this organism.[1]

References

  1. Electrical and optical monitoring of alpha-latrotoxin action at Drosophila neuromuscular junctions. Umbach, J.A., Grasso, A., Zurcher, S.D., Kornblum, H.I., Mastrogiacomo, A., Gundersen, C.B. Neuroscience (1998) [Pubmed]
 
WikiGenes - Universities