Cloning, characterization and expression of beta-N-acetylglucosaminidase gene from Streptomyces thermoviolaceus OPC-520(1)

Biochim Biophys Acta. 1998 Oct 23;1425(2):437-40. doi: 10.1016/s0304-4165(98)00098-1.

Abstract

The nagB gene encoding beta-N-acetylglucosaminidase from S. thermoviolaceus OPC-520 was cloned and sequenced. The nagB gene could encode a protein of 541 amino acids with a calculated molecular mass of 58274. NagB revealed significant similarities to beta-N-acetylhexosaminidases and chitobiases from bacteria, which are classified into family 20 glycosyl hydrolases. NagB effectively hydrolyzed all of the chitin oligosaccharides from dimer to hexamer.

Publication types

  • Comparative Study

MeSH terms

  • Acetylglucosaminidase / biosynthesis
  • Acetylglucosaminidase / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • Chitin / metabolism
  • Cloning, Molecular
  • Gene Expression Regulation, Enzymologic
  • Molecular Sequence Data
  • Sequence Alignment
  • Streptomyces / enzymology
  • Streptomyces / genetics*

Substances

  • Chitin
  • Acetylglucosaminidase

Associated data

  • GENBANK/AB015350