Dimitrios Tsikas
Institute of Clinical Pharmacology
Hannover Medical School
Hannover
Germany. Electronic address:
Name/email consistency: high
- Glutathione promotes prostaglandin H synthase (cyclooxygenase)-dependent formation of malondialdehyde and 15(S)-8-iso-prostaglandin F(2α). Tsikas, D., Suchy, M.T., Niemann, J., Tossios, P., Schneider, Y., Rothmann, S., Gutzki, F.M., Frölich, J.C., Stichtenoth, D.O. FEBS Lett. (2012)
- Potential problems and pitfalls with the use of S-nitrosoglutathione and other S-nitrosothiols in physiology-oriented basic science. Tsikas, D. J. Physiol. (Lond.) (2012)
- Stable isotope gas chromatography-tandem mass spectrometry determination of aminoethylcysteine ketimine decarboxylated dimer in biological samples. Tsikas, D., Evans, C.E., Denton, T.T., Mitschke, A., Gutzki, F.M., Pinto, J.T., Khomenko, T., Szabo, S., Cooper, A.J. Anal. Biochem. (2012)
- Analytical methods for 3-nitrotyrosine quantification in biological samples: the unique role of tandem mass spectrometry. Tsikas, D. Amino. Acids (2012)
- Measurement of nitrite in urine by gas chromatography-mass spectrometry. Tsikas, D., Suchy, M.T., Mitschke, A., Beckmann, B., Gutzki, F.M. Methods Mol. Biol. (2012)
- Measurement of 3-nitro-tyrosine in human plasma and urine by gas chromatography-tandem mass spectrometry. Tsikas, D., Mitschke, A., Gutzki, F.M. Methods Mol. Biol. (2012)
- Comment on: Ott et al. Reduction in Basal Nitric Oxide Activity Causes Albuminuria. Diabetes 2011;60:572-576. Tsikas, D., Engeli, S., Tank, J., Stichtenoth, D.O., Jordan, J. Diabetes (2011)
- GC-MS and HPLC methods for peroxynitrite (ONOO- and O15NOO-) analysis: a study on stability, decomposition to nitrite and nitrate, laboratory synthesis, and formation of peroxynitrite from S-nitrosoglutathione (GSNO) and KO2. Tsikas, D. Analyst (2011)
- Simultaneous gas chromatography-tandem mass spectrometry quantification of symmetric and asymmetric dimethylarginine in human urine. Tsikas, D., Beckmann, B., Gutzki, F.M., Jordan, J. Anal. Biochem. (2011)
- Analysis of NO and its metabolites by mass spectrometry. Comment on 'Detection of nitric oxide in tissue samples by ESI-MS' by Z. Shen, A. Webster, K. J. Welham, C. E. Dyer, J. Greenman and S. J. Haswell. Tsikas, D., Sandmann, J., Beckmann, B. Analyst (2011)
- Quantitative analysis of biomarkers, drugs and toxins in biological samples by immunoaffinity chromatography coupled to mass spectrometry or tandem mass spectrometry: A focused review of recent applications. Tsikas, D. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2010)
- Evidence by gas chromatography-mass spectrometry of ex vivo nitrite and nitrate formation from air nitrogen oxides in human plasma, serum, and urine samples. Tsikas, D., Mitschke, A., Gutzki, F.M., Engeli, S., Jordan, J. Anal. Biochem. (2010)
- Simultaneous measurement of [15N]nitrate and [15N]nitrite enrichment and concentration in urine by gas chromatography mass spectrometry as pentafluorobenzyl derivatives. Tsikas, D., Schwarz, A., Stichtenoth, D.O. Anal. Chem. (2010)
- Identifying and quantifying contaminants contributing to endogenous analytes in gas chromatography/mass spectrometry. Tsikas, D. Anal. Chem. (2010)
- Gas chromatography-mass spectrometry analysis of nitrite in biological fluids without derivatization. Tsikas, D., Böhmer, A., Mitschke, A. Anal. Chem. (2010)
- Quantification of carbonate by gas chromatography-mass spectrometry. Tsikas, D., Chobanyan-Jürgens, K. Anal. Chem. (2010)
- GC-MS determination of creatinine in human biological fluids as pentafluorobenzyl derivative in clinical studies and biomonitoring: Inter-laboratory comparison in urine with Jaffé, HPLC and enzymatic assays. Tsikas, D., Wolf, A., Mitschke, A., Gutzki, F.M., Will, W., Bader, M. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2010)
- A proposal for comparing methods of quantitative analysis of endogenous compounds in biological systems by using the relative lower limit of quantification (rLLOQ). Tsikas, D. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2009)
- Specific GC-MS/MS stable-isotope dilution methodology for free 9- and 10-nitro-oleic acid in human plasma challenges previous LC-MS/MS reports. Tsikas, D., Zoerner, A., Mitschke, A., Homsi, Y., Gutzki, F.M., Jordan, J. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2009)
- GC-MS analysis of S-nitrosothiols after conversion to S-nitroso-N-acetyl cysteine ethyl ester and in-injector nitrosation of ethyl acetate. Tsikas, D., Dehnert, S., Urban, K., Surdacki, A., Meyer, H.H. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2009)
- Nitro-fatty acids occur in human plasma in the picomolar range: a targeted nitro-lipidomics GC-MS/MS study. Tsikas, D., Zoerner, A.A., Mitschke, A., Gutzki, F.M. Lipids (2009)
- De novo synthesis of trideuteromethyl esters of amino acids for use in GC-MS and GC-tandem MS exemplified for ADMA in human plasma and urine: standardization, validation, comparison and proof of evidence for their aptitude as internal standards. Tsikas, D. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2009)
- A critical review and discussion of analytical methods in the L-arginine/nitric oxide area of basic and clinical research. Tsikas, D. Anal. Biochem. (2008)
- Determination of asymmetric dimethylarginine in biological fluids: a paradigm for a successful analytical story. Tsikas, D. Curr. Opin. Clin. Nutr. Metab. Care (2008)
- Accurate quantification of dimethylamine (DMA) in human urine by gas chromatography-mass spectrometry as pentafluorobenzamide derivative: evaluation of the relationship between DMA and its precursor asymmetric dimethylarginine (ADMA) in health and disease. Tsikas, D., Thum, T., Becker, T., Pham, V.V., Chobanyan, K., Mitschke, A., Beckmann, B., Gutzki, F.M., Bauersachs, J., Stichtenoth, D.O. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2007)
- Analysis of nitrite and nitrate in biological fluids by assays based on the Griess reaction: appraisal of the Griess reaction in the L-arginine/nitric oxide area of research. Tsikas, D. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2007)
- Recent methodological advances in the mass spectrometric analysis of free and protein-associated 3-nitrotyrosine in human plasma. Tsikas, D., Caidahl, K. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2005)
- Unusual collision-induced dissociation of fluorated and non-fluorated alpha-nitrotoluene analogs in a gas chromatograph triple-stage quadrupole mass spectrometer under electron-capturing negative-ion chemical ionization conditions. Tsikas, D., Schwedhelm, E., Frölich, J.C. J. Chromatogr. A (2005)
- Methods of quantitative analysis of the nitric oxide metabolites nitrite and nitrate in human biological fluids. Tsikas, D. Free Radic. Res. (2005)
- Simplified HPLC method for urinary and circulating creatinine. Tsikas, D., Wolf, A., Frölich, J.C. Clin. Chem. (2004)
- Gas chromatography-mass spectrometry of cis-9,10-epoxyoctadecanoic acid (cis-EODA). II. Quantitative determination of cis-EODA in human plasma. Tsikas, D., Mitschke, A., Gutzki, F.M., Meyer, H.H., Frölich, J.C. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2004)
- Mass spectrometry-validated HPLC method for urinary nitrate. Tsikas, D. Clin. Chem. (2004)
- Accurate quantification of basal plasma levels of 3-nitrotyrosine and 3-nitrotyrosinoalbumin by gas chromatography-tandem mass spectrometry. Tsikas, D., Schwedhelm, E., Stutzer, F.K., Gutzki, F.M., Rode, I., Mehls, C., Frölich, J.C. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2003)
- Gas chromatography-mass spectrometry of cis-9,10-epoxyoctadecanoic acid (cis-EODA). I. Direct evidence for cis-EODA formation from oleic acid oxidation by liver microsomes and isolated hepatocytes. Tsikas, D., Sawa, M., Brunner, G., Gutzki, F.M., Meyer, H.H., Frölich, J.C. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2003)
- Divergence in urinary 8-iso-PGF(2alpha) (iPF(2alpha)-III, 15-F(2t)-IsoP) levels from gas chromatography-tandem mass spectrometry quantification after thin-layer chromatography and immunoaffinity column chromatography reveals heterogeneity of 8-iso-PGF(2alpha). Possible methodological, mechanistic and clinical implications. Tsikas, D., Schwedhelm, E., Suchy, M.T., Niemann, J., Gutzki, F.M., Erpenbeck, V.J., Hohlfeld, J.M., Surdacki, A., Frölich, J.C. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2003)
- Quantitative determination of circulating and urinary asymmetric dimethylarginine (ADMA) in humans by gas chromatography-tandem mass spectrometry as methyl ester tri(N-pentafluoropropionyl) derivative. Tsikas, D., Schubert, B., Gutzki, F.M., Sandmann, J., Frölich, J.C. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2003)
- Measurement of S-nitrosoalbumin by gas chromatography--mass spectrometry. III. Quantitative determination in human plasma after specific conversion of the S-nitroso group to nitrite by cysteine and Cu(2+) via intermediate formation of S-nitrosocysteine and nitric oxide. Tsikas, D., Sandmann, J., Frölich, J.C. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2002)
- Artifactual-free analysis of S-nitrosoglutathione and S-nitroglutathione by neutral-pH, anion-pairing, high-performance liquid chromatography. Study on peroxynitrite-mediated S-nitration of glutathione to S-nitroglutathione under physiological conditions. Tsikas, D., Denker, K., Frölich, J.C. J. Chromatogr. A (2001)
- Affinity chromatography as a method for sample preparation in gas chromatography/mass spectrometry. Tsikas, D. J. Biochem. Biophys. Methods (2001)
- Electrospray ionization mass spectrometry of low-molecular-mass S-nitroso compounds and their thiols. Tsikas, D., Raida, M., Sandmann, J., Rossa, S., Forssmann, W.G., Frölich, J.C. J. Chromatogr. B Biomed. Sci. Appl. (2000)
- Assessment of nitric oxide synthase activity in vitro and in vivo by gas chromatography-mass spectrometry. Tsikas, D., Sandmann, J., Savva, A., Luessen, P., Böger, R.H., Gutzki, F.M., Mayer, B., Frölich, J.C. J. Chromatogr. B Biomed. Sci. Appl. (2000)
- Endogenous nitric oxide synthase inhibitors are responsible for the L-arginine paradox. Tsikas, D., Böger, R.H., Sandmann, J., Bode-Böger, S.M., Frölich, J.C. FEBS Lett. (2000)
- Solid- and liquid-phase extraction for the gas chromatographic-tandem mass spectrometric quantification of 2,3-dinor-thromboxane B2 and 2,3-dinor-6-oxo-prostaglandin F1 alpha in human urine. Tsikas, D., Gutzki, F.M., Böhme, M., Fuchs, I., Frölich, J.C. J. Chromatogr. A (2000)
- Simultaneous derivatization and quantification of the nitric oxide metabolites nitrite and nitrate in biological fluids by gas chromatography/mass spectrometry. Tsikas, D. Anal. Chem. (2000)
- High-performance liquid chromatographic analysis of nitrite and nitrate in human plasma as S-nitroso-N-acetylcysteine with ultraviolet absorbance detection. Tsikas, D., Rossa, S., Sandmann, J., Frölich, J.C. J. Chromatogr. B Biomed. Sci. Appl. (1999)
- Investigations of S-transnitrosylation reactions between low- and high-molecular-weight S-nitroso compounds and their thiols by high-performance liquid chromatography and gas chromatography-mass spectrometry. Tsikas, D., Sandmann, J., Rossa, S., Gutzki, F.M., Frölich, J.C. Anal. Biochem. (1999)
- Measurement of S-nitrosoalbumin by gas chromatography-mass spectrometry. II. Quantitative determination of S-nitrosoalbumin in human plasma using S-[15N]nitrosoalbumin as internal standard. Tsikas, D., Sandmann, J., Gutzki, F.M., Stichtenoth, D.O., Frölich, J.C. J. Chromatogr. B Biomed. Sci. Appl. (1999)
- Gas chromatographic-mass spectrometric detection of S-nitroso-cysteine and S-nitroso-glutathione. Tsikas, D., Sandmann, J., Rossa, S., Gutzki, F.M., Frölich, J.C. Anal. Biochem. (1999)
- Determination of S-nitrosoglutathione in human and rat plasma by high-performance liquid chromatography with fluorescence and ultraviolet absorbance detection after precolumn derivatization with o-phthalaldehyde. Tsikas, D., Sandmann, J., Holzberg, D., Pantazis, P., Raida, M., Frölich, J.C. Anal. Biochem. (1999)
- Gas chromatographic-tandem mass spectrometric quantification of human plasma and urinary nitrate after its reduction to nitrite and derivatization to the pentafluorobenzyl derivative. Tsikas, D., Gutzki, F.M., Sandmann, J., Schwedhelm, E., Frölich, J.C. J. Chromatogr. B Biomed. Sci. Appl. (1999)
- Inhibition of platelet aggregation by S-nitroso-cysteine via cGMP-independent mechanisms: evidence of inhibition of thromboxane A2 synthesis in human blood platelets. Tsikas, D., Ikic, M., Tewes, K.S., Raida, M., Frölich, J.C. FEBS Lett. (1999)
