Synonyms: FLJ34969 , hypothetical protein LOC201627¹¹

Accession Number: NM_152678 (mRNA)¹¹ and NP_689891 (Protein)¹¹

Summary: FAM116A   is 4679 bp gene present in humans and has conserved   orthologs from P.troglodytes to O. sativa. The gene is located on the short arm of chromosome 3 at position 3p14.3. The mRNA of FAM116A contains an open reading frame coding for 20   exons and 608 amino acids. The FAM116A gene has a conserved and known differentially expressed in neoplastic vs normal cells (DENN) domain of unknown function that is, according to Marchler-Bauer et al., a domain which occurs in several proteins involved in Rab-mediated processes or regulation of MAPK signaling pathways^1,10. The function of the protein is not yet well understood by the scientific community.

Gene Neighborhood and Location:

Gene neighborhood of FAM116A on chromosome 3; Location: 3p14.3²

FAM116A is located on the short arm of chromosome 3 at position 3p14.3⁵

Orthologs:

FAM116A has conserved othologs in mammals, birds, amphibians, fish, invertebrates, insects, and plants. All of the orthologs contain a conserved DENN Domain. FAM116A has no known paralogs.

Postranslational Modifications:

Serine 127 is posttranslationally phosphorylated⁴.

Potential Protein interactions: In the Anopheles   gambiae FAM116A homologous protein a PTZ00009 domain, which codes for Heat Shock Protein 70 Kda (Hsp70), was bound to the FAM116A/DENN domain of the protein^1,10. This phenomenon is what Reiner Veitia describes as the Rosetta   Stone method of protein interaction³. Future studies between the proteins may be fruitful for discovery of the function of the FAM116A protein.

Connection with Heat Shock:

1. Rosetta Stone Hsp70 Protein Interaction with FAM116A in Anopheles gambiae¹⁰
2. Up-regulation of FAM116A in Mus musculus over time in response to heat shock^8,9
3. Relation of the DENN Domain to MAP Kinases, which respond to extracellular stimuli including heat shock⁶
4. ‘Heat Shock Factors’ family of ubiquitously expressed transcription factor predicted by MatInspector⁷

References:

1. CDD: a Conserved Domain Database for the functional annotation of proteins. Marchler-Bauer A et al. Nucleic Acids Res.39(D)225-9. (2011)
2. Gene: Genes and mapped phenotypes. National Center for Biotechnology Information (NCBI).  
3. Rosetta Stone proteins: “chance and necessity”?. Veitia R. Genome Biology, 3(2). (2002)
4. Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Olsen JV. Sci Signal 3. (2010)
5. Genecards [ 
6. Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Pearson G, Robinson F, Beers Gibson T, Xu BE, Karandikar M, Berman K, Cobb MH Endocr. Rev. 22 (2): 153–83. (2001)
7. Genomatix (MatInspector) [ 
8. The role of heat shock transcription factor 1 in the genome-wide regulation of the mammalian heat shock response. Trinklein ND, Murray JI, Hartman SJ, Botstein D et al. Mol Biol Cell. 15(3):1254-61. (2004)
9. National Center for Biotechnology Information. (GeProfiles) [ 
10. National Center for Biotechnology Information. (Homologene) [ 
11. National Center for Biotechnology Information (NCBI) [