High impact information on Cpt2

• Cpt2 mapped to Chr 4 near the locus Pmv19 [1].
• Single-cell quantitative RT-PCR analysis of Cpt1b and Cpt2 gene expression in mouse antral oocytes and in preimplantation embryos [2].
• As for preimplantation embryos, MII oocytes show two groups of Cpt2 expression [2].
• Cpt2 transcripts decrease following fertilization to undetectable levels and are present again later at the morula stage [2].
• Based on the number of Cpt2 transcripts detected, we could discriminate the presence of two groups of embryos with high and low number of transcripts, from the zygote throughout preimplantation development [2].

Anatomical context of Cpt2

• Antral oocytes, classified according to their chromatin configuration in SN (surrounded nucleolus, in which the nucleolus is surrounded by a rim of Hoechst-positive chromatin) and NSN (not surrounded nucleolus, in which this rim is absent), show three groups with different numbers of Cpt2 transcripts [2].
• The degradation of fatty acids occurs in mitochondria and is catalyzed by several carnitine acyl transferases, including two carnitine palmitoyl transferases, CPT-I and CPT-II [2].

Other interactions of Cpt2

• These results show that transcription of both Cpt1b and Cpt2 is triggered at the morula stage, concomitantly with known increasing profiles of oxygen uptake and fatty acids oxidation [2].

Analytical, diagnostic and therapeutic context of Cpt2

• On Northern blot analysis a rat liver CPT II cDNA probe detected a single approximately 2.5-kilobase mRNA in all rat and mouse tissues examined [3].

References