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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

Polr2j  -  polymerase (RNA) II (DNA directed)...

Mus musculus

Synonyms: 14.5kDa, DNA-directed RNA polymerase II subunit J, DNA-directed RNA polymerase II subunit RPB11, Polr2i, RNA polymerase II 13.3 kDa subunit, ...
 
 
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High impact information on Polr2j

  • By means of the yeast two-hybrid system using the 40-kDa subunit of mouse RNA polymerase I, mRPA40, as the bait, we isolated a mouse cDNA which encoded a protein with significant homology in amino acid sequence to the 12.5-kDa subunit of Saccharomyces cerevisiae RNA polymerase II, B12.5 (RPB11) [1].
 

Analytical, diagnostic and therapeutic context of Polr2j

  • Based on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot results, purified recombinant caltrin-His was ascertained to be approximately 14.5kDa [2].

References

  1. Isolation and characterization of cDNA encoding mouse RNA polymerase II subunit RPB14. Nishi, Y., Yamamoto, K., Yao, Y., Yamamoto, M., Nogi, Y., Matsuo, H., Muramatsu, M. Gene (1997) [Pubmed]
  2. Expression of caltrin in the baculovirus system and its purification in high yield and purity by cobalt (II) affinity chromatography. Phan, T.C., Nowak, K.J., Akkari, P.A., Zheng, M.H., Xu, J. Protein Expr. Purif. (2003) [Pubmed]
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