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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

Vps72  -  vacuolar protein sorting 72 (yeast)

Mus musculus

Synonyms: Protein YL-1, Tcfl1, Transcription factor-like 1, Vacuolar protein sorting-associated protein 72 homolog, YL-1, ...
 
 
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High impact information on Vps72

  • Next, we microinjected affinity purified antibodies specific for Glu tubulin (polyclonal SG and monoclonal 4B8) and specific for Tyr tubulin (polyclonal W2 and monoclonal YL1/2) into 3T3 cells [1].
  • Electron microscopy as well as direct and indirect immunofluorescence microscopy shows that YL 1/2 binds to the surface of microtubules polymerized in vitro and in vivo [2].
  • (b) YL 1/2, when injected at intermediate and high concentrations, arrested cells in mitosis [3].
  • In vivo studies using Swiss 3T3 fibroblasts injected with YL 1/2 show that; when injected at low concentration (2 mg IgG/ml in the injection solution), the antibody binds to microtubules without changing their distribution in the cytoplasm [2].
  • These results are discussed in terms of the participation of microtubules in cellular activities such as cell movement and cytoplasmic organization and in terms of the specificity of YL 1/2 for the tyrosylated form of alpha-tubulin [3].
 

Biological context of Vps72

  • YL-1 was also found to undergo unconventional splicing using non-canonical splice sites within exons (intraexonic splicing) to produce several alternative transcripts [4].
 

Anatomical context of Vps72

  • They showed little or no change in distribution and organization during observation, even though these microtubule structures appeared not to be stabilized by injected YL 1/2 since they were readily depolymerized by colcemid or cold treatment and repolymerized upon drug removal or rewarming to 37 degrees C, respectively [3].
  • (c) Injection of an intermediate concentration of YL 1/2 that stopped saltatory movement caused little or no aggregation of intermediate filaments and no dispersion of the Golgi complex [3].
 

Analytical, diagnostic and therapeutic context of Vps72

  • We compared the distribution of a mitosis-activated calcium transport system with that of the microtubule network, by immunofluorescence, using two monoclonal antibodies, one directed against a component of the calcium transport system (7/13), and the other against the common tyrosinated form of alpha-tubulin (YL1/2) [5].

References

  1. Stable, detyrosinated microtubules function to localize vimentin intermediate filaments in fibroblasts. Gurland, G., Gundersen, G.G. J. Cell Biol. (1995) [Pubmed]
  2. A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. I. Biochemical characterization, effects on microtubule polymerization in vitro, and microtubule polymerization and organization in vivo. Wehland, J., Willingham, M.C., Sandoval, I.V. J. Cell Biol. (1983) [Pubmed]
  3. A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. II. Effects on cell movement, organization of microtubules, and intermediate filaments, and arrangement of Golgi elements. Wehland, J., Willingham, M.C. J. Cell Biol. (1983) [Pubmed]
  4. Genomic organization of Tropomodulins 2 and 4 and unusual intergenic and intraexonic splicing of YL-1 and Tropomodulin 4. Cox, P.R., Siddique, T., Zoghbi, H.Y. BMC Genomics (2001) [Pubmed]
  5. Microtubule tracks can be detected in mouse oocytes with an antibody directed against a calcium transporter. de Pennart, H., Cibert, C., Petzelt, C., Maro, B. J. Cell. Sci. (1994) [Pubmed]
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