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Gene Review:

pUB110_p02 - bleomycin resistance protein

Plasmid pUB110

Gennimata, D. et al., Sugiyama, M. et al., Schwarz, S. et al., Semon, D. et al.

Sugiyama, Yuasa, Bhuiyan, Iwai, Masumi, Ueda,

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Disease relevance of pUB110_p02

Bleomycin resistance in Staphylococcus aureus clinical isolates [1].
A DNA sequence containing an open reading frame of 405 bp was subcloned into several expression vectors and bleomycin resistance was expressed at high level in Escherichia coli under the control of lambda PL promoter [2].
Plasmid pSTS7 showed partial restriction map and sequence homology to the previously described tetracycline resistance plasmid pNS1981 from Bacillus subtilis and to the kanamycin/neomycin/bleomycin resistance plasmid pUB110 from S. aureus [3].

High impact information on pUB110_p02

DNA dot-blot hybridization analysis of the bleomycin resistant isolates with an 171 bp probe derived from the plasmid pUB110 indicated that 43 strains (22%) carried pUB110-like bleomycin resistance DNA sequences [1].
Of 13 strains that were analysed by DNA gel electrophoresis and Southern blot DNA hybridization, six were found to carry pUB110-like bleomycin resistance DNA sequences [1].
IS431mec-mediated integration of a bleomycin-resistance gene into the chromosome of a methicillin-resistant Staphylococcus aureus strain isolated in Japan [4].

Biological context of pUB110_p02

We proposed that bleomycin resistance in MRSA B-26 is attributed to the IS431 mec-mediated integration of pUB110 into the chromosome [4].

References

Bleomycin resistance in Staphylococcus aureus clinical isolates. Gennimata, D., Davies, J., Tsiftsoglou, A.S. J. Antimicrob. Chemother. (1996) [Pubmed]
Plasmid-determined bleomycin resistance in Staphylococcus aureus. Semon, D., Movva, N.R., Smith, T.F., el Alama, M., Davies, J. Plasmid (1987) [Pubmed]
A novel plasmid from Staphylococcus epidermidis specifying resistance to kanamycin, neomycin and tetracycline. Schwarz, S., Gregory, P.D., Werckenthin, C., Curnock, S., Dyke, K.G. J. Med. Microbiol. (1996) [Pubmed]
IS431mec-mediated integration of a bleomycin-resistance gene into the chromosome of a methicillin-resistant Staphylococcus aureus strain isolated in Japan. Sugiyama, M., Yuasa, K., Bhuiyan, M.Z., Iwai, Y., Masumi, N., Ueda, K. Appl. Microbiol. Biotechnol. (1996) [Pubmed]

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