High impact information on Polr2f

• Using polypeptides designed to block specific receptor- cofactor or cofactor-cofactor interactions, we show that interactions among ERalpha, its coactivators and the RNA pol II machinery are all required for ERalpha- mediated transcription [1].
• Deletion analysis of TLS-ERG in both mouse L-G myeloid progenitor cells and NIH 3T3 fibroblasts revealed that the RNA Pol II-interacting domain of TLS-ERG resides within the first 173 amino acids [2].
• During Hoxd4 induction, CBP and RNA Pol II were recruited first to the enhancer and then to the promoter [3].
• Whereas the CBP association was transient, RNA Pol II remained associated with both regulatory regions [3].
• Preferential interaction with processive RNA pol II in undamaged cells places BRCA1 in position to link late events in transcription with repair processes in eukaryotic cells [4].

Biological context of Polr2f

• TFIIH is a multiprotein complex that has a central role in the RNA pol II mediated transcription, in DNA repair and in the control of the cell cycle [5].

Anatomical context of Polr2f

• We have generated stable murine cell lines expressing an alpha-amanitin-resistant form of the largest subunit of RNA polymerase II (RNA Pol II LS) [6].

Associations of Polr2f with chemical compounds

• Our results further showed that the transcriptionally active form of RNA Pol II LS containing phosphoserine in position 2 of the CTD repeats was restricted to the nucleus and its levels remained remarkably constant [6].
• In ChIP assays conducted with an antibody to RNA polymerase II (RNA Pol II), the association of RNA Pol II with PEPCK RARE1/RARE2 was significantly decreased in vitamin A deficiency [7].

Analytical, diagnostic and therapeutic context of Polr2f

• Immunofluorescence microscopy studies revealed that monomethylated H4-Lys(20) mainly overlaps with RNA pol II-stained euchromatic regions, thus indicating an association with transcriptionally engaged chromatin [8].

References