High impact information on LRRC8C

• The novel gene fad158, having a transmembrane domain and leucine-rich repeat, stimulates adipocyte differentiation [1].
• Both mouse and human fad158 encode 803 amino acids and contain 4 transmembrane regions and 8 leucine-rich repeat motifs [1].
• Expression of fad158 was induced at an early stage in differentiating 3T3-L1 cells and was observed in the skeletal muscle [1].
• TA-LRRP, AD158, and LRRC5 might be implicated in proliferation and activation of lymphocytes and monocytes [2].
• RNAi-mediated knockdown of fad24 or antisense fad158 inhibited adipogenesis of 3T3-L1 preadipocytes and decreased expressions of PPARgamma and C/EBPalpha [3].

Biological context of LRRC8C

• Since rapid amplification of cDNA end (RACE), cDNA library screening, and a genome database search have revealed that two of these genes are novel, we have named them factor for adipocyte differentiation (fad) 24 and fad158 [3].
• The database research of amino acid sequences revealed that fad24 has a basic leucine zipper motif and an NOC domain, and fad158 has four transmembrane domains and eight leucine-rich repeats [3].

Anatomical context of LRRC8C

• Moreover, it was found that FAD24 localizes in the nucleus, especially within nuclear speckles and nucleolus, and FAD158 localizes to the endoplasmic reticulum (ER) [3].
• Furthermore, the constitutive overexpression of fad24 or fad158 in the mouse fibroblast cell line NIH-3T3 resulted in adipocyte conversion when stimulated with adipogenic inducers and PPARgamma ligand BRL49653 [3].

Associations of LRRC8C with chemical compounds

• The expression of fad24 and fad158 transiently increased after the addition of adipogenic inducers [insulin, dexamethasone, 3-isobutyl-1-methylxanthine, fetal bovine serum (FBS)] [3].

References