Disease relevance of FGF16

• Although the predicted FGF-16 amino acid sequence lacks a typical signal sequence, recombinant rat FGF-16 was efficiently secreted by Sf9 insect cells infected with recombinant baculovirus containing the cDNA [1].

High impact information on FGF16

• In the present report, we confirmed by RT-PCR analysis that FGF18 is wildly expressed in the cardiovascular tissue, while FGF16 showed a more restricted expression pattern [2].
• HUVEC clearly demonstrated chemotaxis towards FGF16 and FGF18 [2].
• Fibroblast growth factor 16 and 18 are expressed in human cardiovascular tissues and induce on endothelial cells migration but not proliferation [2].
• We have also determined the structure of human FGF-16 with high amino acid sequence identity (98.6%) to rat FGF-16 [1].
• Structure and expression of a novel member, FGF-16, on the fibroblast growth factor family [1].

Biological context of FGF16

• Comparative genomics on FGF16 orthologs [3].
• These facts indicate that FGF16, FGF18 and FGF20 are pharmacogenomics targets in the field of oncology and regenerative medicine [3].
• Rat FGF-16 is expressed in brown adipose tissue during embryonic development but also shows some specificity for the postnatal heart [4].
• As a first step towards exploiting mouse genetics in this regard, we have used reverse transcriptase-polymerase chain reaction and primers based on the rFGF-16 sequence to clone the adult mouse (m) FGF-16 cDNA [4].

Anatomical context of FGF16

• However, recombinant FGF16 and FGF18 failed to induce endothelial cell proliferation or sprouting in a three-dimensional in vitro angiogenesis assay [2].

Analytical, diagnostic and therapeutic context of FGF16

• Based on sequence analysis, mFGF-16 and hFGF-16 share at least 95.2 and 99% nucleotide and amino acid similarity, respectively [4].

References