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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Denaturing high-performance liquid chromatography-based genotyping and genetic variation of FUT2 in Sri Lanka.

BACKGROUND: Human ABO-secretor locus (FUT2) is highly polymorphic in many human populations. STUDY DESIGN AND METHODS: The applicability of denaturing high-performance liquid chromatography (DHPLC) analysis was evaluated for genotyping the FUT2 in two Sri Lankan populations (Tamil and Sinhalese). RESULTS: Although DHPLC failed to detect one allele, 302C>T, of eight alleles, this method reduces the number of samples to be sequenced and can detect novel polymorphisms by comparing the elution profiles. CONCLUSION: These results suggest that DHPLC analysis is a useful high-throughput method for genotyping FUT2 and, further, that the genetic backgrounds of two Sri Lankan populations are quite similar, with little genetic flow from neighboring East and Southeast Asian populations to Sri Lanka.[1]

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