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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Cometabolic degradation of chlorinated aromatic compounds.

The degradation of chlorobenzene was investigated with the specially chosen strain Methylocystis sp. GB 14 DSM 12955, using 23 ml headspace vials and in a soil column filled with quaternary aquifer material from a depth of 20 m. A long-term experiment was carried out in this column, situated in a mobile test unit at a contaminated location in Bitterfeld (Germany). Groundwater polluted by chlorobenzene was continuously fed through the column, through which a mixture comprising 4% CH(4) and 96% air was bubbled. Chlorobenzene was oxidized by up to 80% under pure culture conditions in the model experiments and was completely degraded under the mixed culture conditions of the column experiments. Over a period of 4 months, the stability of the biological system was monitored regularly by analyzing the sMMO activity as well as by classical microbiological and molecular biological methods.[1]


  1. Cometabolic degradation of chlorinated aromatic compounds. Jechorek, M., Wendlandt, K.D., Beck, M. J. Biotechnol. (2003) [Pubmed]
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