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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Automated sequential affinity chromatography of sea urchin embryo DNA binding proteins.

An automated method of running a tandem sequence of oligonucleotide affinity columns was used to purify factors that interact specifically with cis-regulatory sites of the CyIIIa cytoskeletal actin gene of the sea urchin embryo (Strongylocentrotus purpuratus). The method allows quantitative enrichment in a single chromatographic run of up to 12 different sequence-specific DNA binding proteins, each of which may then be readily purified to homogeneity by methods such as preparative gel electrophoresis. The affinity chromatography and identification of six different CyIIIa-regulatory factors is described, and the general utility of the method is discussed.[1]

References

  1. Automated sequential affinity chromatography of sea urchin embryo DNA binding proteins. Coffman, J.A., Moore, J.G., Calzone, F.J., Britten, R.J., Hood, L.E., Davidson, E.H. Mol. Marine Biol. Biotechnol. (1992) [Pubmed]
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