Serum half-life of pituitary gonadotropins is decreased by sulfonation and increased by sialylation in women.
CONTEXT: The gonadotropins are secreted from the human pituitary as spectra of isoforms with different degrees of sulfonation and sialylation of the oligosaccharides, modifications suspected to determine their half-lives in the circulation. OBJECTIVES: Our objectives were to determine the isoform composition of the serum gonadotropins during GnRH receptor blockade, and to estimate the half-lives in circulation of isoforms with 0-1-2-3 sulfonated N-acetylgalactosamine (SO(3)-GalNAc) residues. DESIGN/PARTICIPANTS: Serum samples were collected in seven healthy women before and up to 20 h after administration of the NAL-GLU GnRH antagonist. MAIN OUTCOME MEASURES: The number of sialic acid and SO(3)-GalNAc residues per LH and FSH molecule and the distribution of molecules with 0-1-2-3 sulfonated residues were measured. The half-lives were estimated by monoexponential decay. RESULTS: More sialylated and less sulfonated gonadotropin isoforms remain longer in circulation during GnRH receptor blockade. LH isoforms with two and three sulfonated residues per molecule had shorter half-lives compared with those with zero and one (109 and 80 vs. 196 and 188 min; P < 0.01). FSH isoforms with one and two sulfonated residues had shorter half-lives than those with zero (485 and 358 vs. 988 min; P < 0.01). CONCLUSIONS: The decline in LH and FSH during GnRH receptor blockade is associated with a decrease in sulfonated and increase in sialylated residues. The rapid disappearance of LH isoforms with two and three SO(3)-GalNAc residues suggests their removal by hepatic SO(3)-GalNAc-receptors similar to those in rodents. Episodical secretion of spectra of isoforms with different half-lives is expected to lead to continuous changes in gonadotropin isoform compositions in blood.[1]References
- Serum half-life of pituitary gonadotropins is decreased by sulfonation and increased by sialylation in women. Wide, L., Eriksson, K., Sluss, P.M., Hall, J.E. J. Clin. Endocrinol. Metab. (2009) [Pubmed]
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