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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Use of terpene-based solvents (Hemo-De, Histoclear, and Shandon and BDH xylene substitutes) in place of xylene in the Ehrlich indole test.

Although less effective than xylene and Kovacs reagent as indole extractants, Hemo-De and Shandon xylene substitute can be used in the Ehrlich indole test if a less toxic solvent is desirable. In doubtful cases, xylene should be used. Because of the blocking action shown in the test, use of indole-nitrite broth cannot be recommended.[1]


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