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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

High-performance liquid chromatographic determination of a naturally occurring xylosyl thioether, 9-[5'-deoxy-5'(methylthio)-beta-D-xylofuranosyl]adenine.

A high-performance liquid chromatographic (HPLC) method for the separation and purification of 9-[5'-deoxy-5'(methylthio)-beta-D-xylofuranosyl]adenine (xylosyl-MTA), a naturally occurring analogue of 5'-deoxy-5'-methylthioadenosine (MTA), has been developed. The compound was purified from perchloric extracts of biological samples by reversed-phase HPLC on an Ultrasil ODS RP-18 column. Isocratic elution resulted in a complete separation between the xylosyl thioether and MTA. The determination of xylosyl-MTA in the nudibranch mollusc Doris verrucosa was also performed. Its concentration ranged from 330 to 380 nmol/g, considerably exceeding the cellular levels of MTA.[1]


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