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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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High-performance liquid chromatographic separation and partial characterization of human protamines 1, 2, and 3.

A method for separating the three human protamines by HPLC of underivatized, total protamine extracts on a Nucleosil RP- C18 column is described. The identities of the three proteins have been confirmed by a combination of disc gel electrophoresis, amino acid composition, and primary sequence analysis. The results show that human protamine 3 elutes first, closely followed by protamine 2. Protamine 1 elutes later. The amino acid compositions and partial amino terminal sequences of human protamines 2 and 3 indicate that these two proteins are very closely related and suggest that they differ only by three amino-terminal amino acids.[1]

References

  1. High-performance liquid chromatographic separation and partial characterization of human protamines 1, 2, and 3. Balhorn, R., Corzett, M., Mazrimas, J., Stanker, L.H., Wyrobek, A. Biotechnol. Appl. Biochem. (1987) [Pubmed]
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